topic 06. Sequence alignment post-process

Uncertainty and its effect on downstream analysis

​How to detect uncertainty

​How to score MSA?

​Another issue: huge amount data

• Supporting Materials

  1. TCS web server

  2. TCS: Chang, J.-M. M., Di Tommaso, P. & Notredame, C. TCS: a new multiple sequence alignment reliability measure to estimate alignment accuracy and improve phylogenetic tree reconstruction.Mol. Biol. Evol. 31, 1625–37 (2014).